Recombinant Human BCAM Protein(His Tag)

SKU: PDMH100294

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:BCAM

Target Synonym:MSK;BCAM;AU;CD239;LU;MSK19;Auberger B antigen;Basal cell adhesion molecule;B-CAM cell surface glycoprotein;F8/G253 antigen;Lutheran antigen;Lutheran blood group glycoprotein;Rh30CE;Antigen identified by monoclonal F8;B CAM cell surface glycoprotein;B cell adhesion molecule;Basal cell adhesion molecule (Lu and Au blood groups);Basal cell adhesion molecule (Lutheran blood group);Basal cell adhesion molecule Lu and Au blood groups;Basal cell adhesion molecule Lutheran blood group;CD239 antigen;Glycoprotein 95kDa;Lutheran;Lutheran blood group (Auberger b antigen included);Lutheran blood group Auberger b antigen included

Research Areas:Cardiovascular;Immunology

Conjugation:

Target Species:Human

Expression Host:Mammalian

Application:

Fusion tag:C-His

UNIProt ID:P50895

Accession:P50895

Background:The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region , and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells , Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently , BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166 , and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation , adhesion , migration , and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro , thus being a candidate molecule involved in tumor progression. In addition , BCAM interacts with integrin in sickle red cells , and thus may potentially play a role in vaso-occlusive episodes.

Concentration:

Activity:Not validated for activity

Sequence:Glu32-Ala547

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:56.6 kDa

ObservedMW:80 kDa