Recombinant Human ATG5 Protein

SKU: PKSH032101

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: ATG5

Target Synonym: APG5-like;APG5L;ASP;ATG5;Apoptosis-specific protein;Autophagy protein 5

Research Areas: Signal Transduction;Cell biology;Cardiovascular;Cancer;metabolism;

Conjugation:

Target Species: Human

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: Q9H1Y0

Accession: Q9H1Y0

Background: ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Concentration:

Activity: Not validated for activity

Sequence: Met 1-Asp275

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 0.2M NaCl, 1mM DTT, 40% Glycerol, pH 8.0.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 32.4 kDa

ObservedMW: 33 kDa