Recombinant Human Amphiregulin protein

SKU: CD02343

Size:10 ug

Background:Amphiregulin is an EGF related growth factor and was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. It is mainly expressed numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Amphiregulin signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. It also inhibits the growth of certain carcinoma cell lines. Mutations in this encoded protein are associated with a psoriasis-like skin phenotype.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Human Amphiregulin

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is between 5-1 ng/ml.

Synonyms:AR, AREGB, CRDGF, SDGF,Amphiregulin, Human

Endotoxin:Less than 1 EU/μg of rHuAmphiregulin as determined by LAL method.

Storage:-20ºC

Sequence:SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK

uniprot accession:P15514

Molecular weight:Approximately 11.3 kDa, a single non-glycosylated polypeptide chain containing 98 amino acid residues.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.