Recombinant Human Activin RIIA/ACVR2A Protein (Fc & His Tag)

SKU: PKSH032039

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: Activin RIIA;ACVR2A

Target Synonym: ACTR-IIA;ACTRIIA;ACVR2;ACVR2A;Activin Receptor Type IIA;Activin Receptor Type-2A

Research Areas: Signal Transduction;Cancer;metabolism;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Fc-His

UNIProt ID: P27037

Accession: P27037

Background: Activin Receptor Type-2A is a protein that in humans is encoded by the ACVR2A gene. ACVR2A is an activin type 2 receptor. This gene encodes activin A type II receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins; composed of a ligand-binding extracellular domain with cysteine-rich region; a transmembrane domain; and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding; resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases.

Concentration:

Activity: Immobilized Human INHBC-His at 10μg/ml (100 μl/well) can bind Human ACVR2A-Fc-His. The ED₅₀ of Recombinant Human ACVR2A-Fc-His is 2-8 ug/ml.

Sequence: Ala20-Pro134

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 41.2 kDa

ObservedMW: 60kDa&40kDa