Recombinant Human ACE2 Protein (Avi-His Tag)

SKU: PKSR030493

Size: 100μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: ACE2

Target Synonym: ACE-Related Carboxypeptidase;ACE2;ACEH;Angiotensin-Converting Enzyme 2;Angiotensin-Converting Enzyme Homolog;Metalloprotease MPROT15

Research Areas: Cell biology;Cardiovascular

Conjugation: Biotinylated

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Avi-His

UNIProt ID: Q9BYF1

Accession: Q9BYF1

Background: Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.

Concentration:

Activity: Immobilized 2019-nCoV S1 Protein-Fc(Cat#PKSR030480) at 2μg/ml (100 μl/well) can bind Biotinylated Human ACE-2-Avi-His (Cat#PKSR030493). The ED50 of Biotinylated Human ACE-2-Avi-His (Cat#PKSR030493) is 0.17 ug/ml.

Sequence: Gln18-Ser740

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 300mM NaCl, 1mM ZnCl₂, 10% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 86.2 kDa

ObservedMW: 110-140 kDa