Recombinant Human 4-1BB/TNFRSF9 Protein (Fc Tag)

SKU: PKSH032026

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: 4-1BB;TNFRSF9

Target Synonym: 4-1BB ligand receptor;CD137;CDw137;ILA;T-cell antigen 4-1BB homolog;T-cell antigen ILA;TNFRSF9

Research Areas: Cell biology;Cancer;immunology;Stem cells

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-Fc

UNIProt ID: Q07011

Accession: Q07011

Background: Tumor necrosis factor receptor superfamily member 9(TNFRSF9); also known as CD137 and 4-1BB; is an inducible T cell surface protein belonging to the tumor necrosis factor receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. CD137 is expressed by mesenchymal cells; including endothelial cells; chondrocytes; and cells of the central nervous system. CD137 is also broadly expressed by cells of the human immune system; is broadly expressed by cells of the human immune system; including activated CD8+ and CD4+ T cells; activated natural killer (NK) cells; follicular dendritic cells (FDCs) and monocytes. CD137 has diverse roles in the immune response; the one key function is to promote the survival of both T cells and dendritic cells by binding the cognate ligand CD137L (4-1BBL).

Concentration:

Activity: Immobilized Human 4-1BBL-His(Cat: PKSH032023) at 10 μg/ml(100 μl/well) can bind Human 4-1BB-Fc. The ED₅₀ of Human 4-1BB-Fc is 16. 8 ng/ml.

Sequence: Leu24-Gln186

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 44.2 kDa

ObservedMW: 58 kDa