Recombinant Histone H3 (Di Methyl Lys14) Monoclonal Antibody

SKU: AN302108L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Histone H3 (Di Methyl Lys14)

Target Synonym:Histone H;H3C;H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A;H3C1;Histone H3.1;Histone H3/a;Histone H3/b;H3FL;HIST1H3B;H3FC;HIST1H3C;H3FB;HIST1H3D;H3FD;HIST1H3E;H3FI;HIST1H3F;H3FH;HIST1H3G;H3FK;HIST1H3H;H3FF;HIST1H3I;H3FJ;HIST1H3J;H3/A;H3FA;H3FB;H3FC;H3FD;H3FF;H3FH;H3FI;H3FJ;H3FK;H3FL;Hist1h3a;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;Histone H3.1;Histone H3/a;Histone H3/b;H3 histone family;H31;H3a;histone 1;Histone cluster 1;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;member A

Research Areas:Epigenetics and Nuclear Signaling;Isotype;Loading Controls

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat;Mouse

Application:WB;IHC;IF;ChIP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A832

UNIProt ID:P68431

Accession:

Background:Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. histone methyltransferases (HMTs) and histone demethylases (HDMs) are major regulating factors.

Concentration:1 mg/mL

Immunogen:Dimethylated human histone H3 (Lys14) peptide

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:2000;IHC 1:100-1:500;IF 1:50;ChIP 6 μg/5×10⁶ cells

Calculated MW:15 kDa

ObservedMW:15 kDa