Recombinant GFP Protein

SKU: PDEO100021

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:GFP

Target Synonym:Green fluorescent protein

Research Areas:Tags & Cell Markers

Conjugation:

Target Species:Aequorea victoria

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P42212

Accession:P42212

Background:Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Lys238

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4, 15% glycerol, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:26.9 kDa

ObservedMW:30 kDa