Recombinant Cyclin B1 Monoclonal Antibody

SKU: AN301834L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:Cyclin B1

Target Synonym:G2 mitotic specific cyclin B;G2/mitotic-specific cyclin-B;Cyclin B;CCNB;CCNB1;Cyclin B1;CCNB 1;G2 mitotic specific cyclin B1;G2/mitotic-specific cyclin-B1

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling;Cancer

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IF;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A546

UNIProt ID:P14635

Accession:

Background:Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.

Concentration:1 mg/mL

Immunogen:Recombinant human Cyclin B1 fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IF 1:50;IP 1:50-1:100

Calculated MW:55 kDa

ObservedMW:55 kDa