Recombinant Clostridium perfringens Perfringolysin O(pfo)

Recombinant Clostridium perfringens Perfringolysin O(pfo)

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>Several Other Sizes Are Also Available. Please Inquire. Default Size: 200ug

Updated Date: Stock Protein updated on 20170405

Research areas: Microbiology

Target / Protein: pfo

Biologically active: Not Tested

Expression system: E.coli

Species of origin: Clostridium perfringens (strain 13 / Type A)

Delivery time: 3-7 business days

Uniprot ID: P0C2E9


Tag info: N-terminal 6xHis-SUMO-tagged

Expression Region: 29-500aa

Protein length: Full Length of Mature Protein

MW: 68.7 kDa

Alternative Name(s): Theta-toxin Thiol-activated cytolysin

Relevance: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.

Reference: "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002)

Purity: Greater than 90% as determined by SDS-PAGE.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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