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Updated Date: Stock Protein updated on 20170405
Research areas: others
Target / Protein:
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Chlamydomonas reinhardtii (Chlamydomonas smithii)
Delivery time: 3-7 business days
Uniprot ID: P31178
AA Sequence: EIYAGKPIDLRTIVYIMDFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERRRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQ
Tag info: N-terminal 6xHis-SUMO-tagged
Expression Region: 184-638AA
Protein length: Full Length of Mature Protein
MW: 65.6 kDa
Alternative Name(s): Gamete lytic enzyme
Relevance: Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion. This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.
Reference: "Primary structure and expression of a gamete lytic enzyme in Chlamydomonas reinhardtii: similarity of functional domains to matrix metalloproteases." Kinoshita T., Fukuzawa H., Shimada T., Saito T., Matsuda Y. Proc. Natl. Acad. Sci. U.S.A. 89:4693-4697(1992)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.