Recombinant CD3G Monoclonal Antibody

SKU: AN301477L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies

Abbreviation:CD3G

Target Synonym:T3G;CD3G;T3;CD_antigen: CD3g;CD3g molecule;CD3-GAMMA;IMD17;T-cell receptor T3 gamma chain;T-cell surface glycoprotein CD3 gamma chain

Research Areas:Immunology;Stem Cells

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:WB;IHC;IF;IP

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A172

UNIProt ID:P09693

Accession:

Background:Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.

Concentration:1 mg/mL

Immunogen:Recombinant human CD3G fragment

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:WB 1:500-1:1000;IHC 1:500-1:2000;IF 1:50;IP 1:50

Calculated MW:20 kDa

ObservedMW:18-28 kDa