Recombinant ASK1 Monoclonal Antibody

SKU: AN300808L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:ASK1

Target Synonym:ASK;MEKK;MAP3K;MAPKKK;MAP3K5;ASK1;MAPKKK5;MEKK5;Apoptosis signal regulating kinase 1;Apoptosis signal-regulating kinase 1;ASK 1;ASK-1;M3K5;MAP/ERK kinase kinase 5;MAPK/ERK kinase kinase 5;MEK kinase 5;MEKK 5;Mitogen activated protein kinase kinase kinase 5;mitogen-activated protein kinase kinase kinase 5

Research Areas:Cell Biology;Cardiovascular

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Mouse

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:4B7

UNIProt ID:Q99683

Accession:

Background:Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Concentration:0.2 mg/mL

Immunogen:Recombinant Human ASK1 protein

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A

Dilution:WB 1:1000-1:5000

Calculated MW:155 kDa

ObservedMW:155 kDa