Rat TOP2(Topoisomerase II) ELISA Kit

SKU: ELK4458

Size:96T

Reactivity:Rat

Alternative Names:DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

Assay Type:Sandwich

Sensitivity:0.064 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;Tumor immunity;Developmental science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TOP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TOP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TOP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TOP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P41516