Rat Sterol 26-hydroxylase, mitochondrial,CYP27A1 ELISA KIT

SKU: E3344Ra

Size:96 Tests-1 Kit

Background:Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism. Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation. Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium. May play a redundant role in vitamin D biosynthesis (By similarity). Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (PubMed:2175615). {ECO:0000250|UniProtKB:Q02318, ECO:0000269|PubMed:2175615}.

Research_area:

Applications:ELISA

Target_protein:Cyp27a1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.92ng/ml

Detection range:1.88-120ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P17178

MASS(Da):60733

GeneID:301517

Synonyms:Cyp27a1 Cyp27

Gene names:Cyp27a1

Standard solution:240ng/ml