Rat LN(Laminin) Microsample ELISA Kit

SKU: ELK10526MS

Size:96T

Reactivity:Rat

Alternative Names:LAM

Assay Type:Sandwich

Sensitivity:1.09 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Signal Transduction

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LN in the samples is then determined by comparing the OD of the samples to the standard curve.

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