Rat Lanosterol 14 Alpha Demethylase, CYP51A1 ELISA Kit

SKU: E1518Ra

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid (PubMed:7665087, PubMed:10544287, PubMed:11328599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:7665087, PubMed:10544287, PubMed:11328599).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:Cyp51a1

Reactivity:Rat

Assay type:Sandwich

Sensitivity:0.026ng/ml

Detection range:0.05-20ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Lanosterol 14 alpha demethylase

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q64654

MASS(Da):56,707

GeneID:25427

Synonyms:Cyp51a1;CYPLI;Cytochrome P450 51A1;Cytochrome P45014DM;Cytochrome P450-14DM;Cytochrome P450LI;Lanosterol 14-alpha demethylase;LDM;RATCP14DM;Sterol 14-alpha demethylase

Gene names:Cyp51a1

Standard solution:24ng/ml