Rat Glyceraldehyde-3-phosphate Dehydrogenase, GAPDH ELISA Kit

SKU: E1481Ra

Size:96 Tests-1 Kit

Background:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity).

Research_area:Cancer

Applications:ELISA

Target_protein:GAPDH

Reactivity:Rat

Assay type:Sandwich

Sensitivity:7.42ng/L

Detection range:15-3800ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Glyceraldehyde-3-Phosphate Dehydrogenase

Species:Rat

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P04797

MASS(Da):35,828

GeneID:10835113724383

Synonyms:38 kDa BFA-dependent ADP-ribosylation substrate;BARS-38;GAPDH;Glyceraldehyde-3-phosphate dehydrogenase;Peptidyl-cysteine S-nitrosylase GAPDH

Gene names:GAPDH

Standard solution:4000ng/L