Rat cAMP(Cyclic Adenosine Monophosphate) ELISA Kit

SKU: ELK0754

Size:96T

Reactivity:Rat

Alternative Names:c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate

Assay Type:Competitive Inhibition

Sensitivity:143.3 pg/mL

Standard:20000 pg/mL

Detection range:312.5-20000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2.5h

Research Area:Signal transduction;Metabolic pathway;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat cAMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat cAMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.

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