Rat C3a(Complement Component 3a) Microsample ELISA Kit

SKU: ELK10299MS

Size:96T

Reactivity:Rat

Alternative Names:Complement Component 3a

Assay Type:Sandwich

Sensitivity:2.01 ng/mL

Standard:500 ng/mL

Detection range:7.81-500 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Infection immunity;Immune molecule;Autoimmunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C3a in the samples is then determined by comparing the OD of the samples to the standard curve.

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