Rat bCTx(Beta-Crosslaps) ELISA Kit

SKU: ELK1546

Size:96T

Reactivity:Rat

Alternative Names:Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction

Assay Type:Competitive Inhibition

Sensitivity:39.1 pg/mL

Standard:8000 pg/mL

Detection range:125-8000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.

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