Rat ACV-A(Activin A) ELISA Kit

SKU: ELK0175

Size:96T

Reactivity:Rat

Alternative Names:ACVA; Activin Beta A Beta A Homodimer

Assay Type:Sandwich

Sensitivity:4.57 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Endocrinology;Reproductive science;Hormone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.

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