Rabbit APOE(Apolipoprotein E) ELISA Kit

SKU: ELK11093

Size:96T

Reactivity:Rabbit

Alternative Names:Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

Assay Type:Competitive Inhibition

Sensitivity:0.25 µg/mL

Standard:50 µg/mL

Detection range:0.78-50 µg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Cardiovascular biology;Hepatology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit APOE in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P18287