This product uses a unique lysis buffer system to quickly release RNA from cultured cell samples for use in RT-qPCR reactions, eliminating RNA purification process. The required RNA template can be obtained in just 7 minutes in cell culture plates. Provided with reagents the 5× Direct RT Mix and 2× Direct qPCR Mix -SYBR, you can quickly and effectively obtain real-time quantitative PCR results from a cell culture plate in three hours.
For cell direct RT-qPCR using 10-1,000,000 cells.
Kit composition |
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Kit composition (20 μl qPCR reaction system) |
DRT-01011 |
DRT-01012 |
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200 T |
1000 T |
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Part I Cell Lysis |
Buffer CL |
4 ml |
20 ml |
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Foregene Protease Plus II |
80 μl |
400 μl |
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Buffer ST |
400 μl |
1 ml × 2 |
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Part II RT qPCR |
DNA Eraser |
80 μl |
400 μl |
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5× Direct RT Mix |
160 μl |
800 μl |
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2× Direct qPCR Mix-SYBR |
1 ml × 2 |
1.7 ml × 6 |
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50× ROX Reference Dye |
40 μl |
200 μl |
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RNase-Free ddH2O |
1.7 ml |
10 ml |
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