Purified Rabbit anti-VPS4A Antibody

SKU: DL98137A

Size 1: 100 ul

Synonyms : VPS4; Vacuolar protein sorting-associated protein 4A; Protein SKD2; VPS4-1; hVPS4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to VPS4A

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human VPS4A

WB description: Western blot analysis of VPS4A expression in MCF7 (A), Hela (B), mouse brain (C), rat brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of VPS4A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of VPS4A staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 27183

Gene ID (Mouse): 116733

Gene ID(Rat): 246772

SwissProt (Human): Q9UN37

SwissProt (Mouse): Q8VEJ9

SwissProt (Rat): Q793F9

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.