Purified Rabbit anti-Villin Antibody

SKU: DL95289A

Size 1: 100 ul

Synonyms : VIL; Villin-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, B, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Villin

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Villin. The exact sequence is proprietary.

WB description: Western blot analysis of Villin expression in K562 (A), HT29 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Villin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200)

Gene ID(Human): 7429

Gene ID (Mouse): 22349

Gene ID(Rat):

SwissProt (Human): P09327

SwissProt (Mouse): Q62468

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.