Purified Rabbit anti-UBE2J2 Antibody

SKU: DL96434A

Size 1: 100 ul

Synonyms : NCUBE2; Ubiquitin-conjugating enzyme E2 J2; Non-canonical ubiquitin-conjugating enzyme 2; NCUBE-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to UBE2J2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human UBE2J2

WB description: Western blot analysis of UBE2J2 expression in Hela (A), mouse testis (B), mouse brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of UBE2J2 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of UBE2J2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 118424

Gene ID (Mouse): 140499

Gene ID(Rat):

SwissProt (Human): Q8N2K1

SwissProt (Mouse): Q6P073

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.