Purified Rabbit anti-UBE2C Antibody

SKU: DL96089A

Size 1: 100 ul

Synonyms : UBCH10; Ubiquitin-conjugating enzyme E2 C; UbcH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to UBE2C

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human UBE2C

WB description: Western blot analysis of UBE2C expression in THP1 (A), SW620 (B), SKOV3 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of UBE2C staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of UBE2C staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 11065

Gene ID (Mouse): 68612

Gene ID(Rat):

SwissProt (Human): O00762

SwissProt (Mouse): Q9D1C1

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.