Purified Rabbit anti-Tyrosine Hydroxylase Antibody

SKU: DL92338A

Size 1: 100 ul

Synonyms : TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, D

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to Tyrosine Hydroxylase

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

WB description: Western blot analysis of IGF1 Receptor (pY1161) expression in HeLa (A), NIH3T3 (B), mouse heart (C) whole cell lysates.

IHC description: Immunohistochemical analysis of IGF1 Receptor (pY1161) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of IGF1 Receptor (pY1161) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 3480; 3643

Gene ID (Mouse): 16001; 16337

Gene ID(Rat): 25718

SwissProt (Human): P08069; P06213

SwissProt (Mouse): Q60751; P15208

SwissProt (Rat): P24062; P15127

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.