Purified Rabbit anti-TC-PTP Antibody

SKU: DL97052A

Size 1: 100 ul

Synonyms : PTPT; Tyrosine-protein phosphatase non-receptor type 2; T-cell protein-tyrosine phosphatase; TCPTP

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to TC-PTP

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human TC-PTP

WB description: Western blot analysis of TC-PTP expression in Jurkat (A), K562 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of TC-PTP staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of TC-PTP staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 5771

Gene ID (Mouse): 19255

Gene ID(Rat): 117063

SwissProt (Human): P17706

SwissProt (Mouse): Q06180

SwissProt (Rat): P35233

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.