Purified Rabbit anti-STS-1 Antibody

SKU: DL97161A

Size 1: 100 ul

Synonyms : KIAA1959; STS1; Ubiquitin-associated and SH3 domain-containing protein B; Cbl-interacting protein p70; Suppressor of T-cell receptor signaling 1; STS-1; T-cell ubiquitin ligand 2; TULA-2; Tyrosine-protein phosphatase STS1/TULA2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to STS-1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human STS-1

WB description: Western blot analysis of STS-1 expression in SW620 (A), SKOV3 (B), mouse spleen (C), mouse lung (D), rat brain (E) whole cell lysates.

IHC description: Immunohistochemical analysis of STS-1 staining in rat spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of STS-1 staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 84959

Gene ID (Mouse): 72828

Gene ID(Rat):

SwissProt (Human): Q8TF42

SwissProt (Mouse): Q8BGG7

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.