Purified Rabbit anti-ST3GAL4 Antibody

SKU: DL96195A

Size 1: 100 ul

Synonyms : CGS23; NANTA3; SIAT4C; STZ; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 4; Alpha 2,3-ST 4; Beta-galactoside alpha-2,3-sialyltransferase 4; Alpha 2,3-sialyltransferase IV; Gal-NAc6S; Gal-beta-1,4-GalNAc-alpha-2,3-sialyltransferase; SAT-3; ST-4; ST3Gal IV; ST3GalIV; ST3GalA.2; STZ; Sialyltransferase 4C; SIAT4-C

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to ST3GAL4

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human ST3GAL4

WB description: Western blot analysis of ST3GAL4 expression in HT29 (A), rat testis (B), rat liver (C) whole cell lysates.

IHC description: Immunohistochemical analysis of ST3GAL4 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of ST3GAL4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Gene ID(Human): 6484

Gene ID (Mouse):

Gene ID(Rat): 363040

SwissProt (Human): Q11206

SwissProt (Mouse):

SwissProt (Rat): P61131

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.