Purified Rabbit anti-Smoothened Antibody

SKU: DL93249A

Size 1: 100 ul

Synonyms : SMOH; Smoothened homolog; SMO; Protein Gx

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to Smoothened

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Smoothened. The exact sequence is proprietary.

WB description: Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.

IHC description: Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 6608

Gene ID (Mouse): 319757

Gene ID(Rat):

SwissProt (Human): Q99835

SwissProt (Mouse): P56726

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.