Purified Rabbit anti-SFRP4 Antibody

SKU: DL97092A

Size 1: 100 ul

Synonyms : FRPHE; Secreted frizzled-related protein 4; sFRP-4; Frizzled protein, human endometrium; FrpHE

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to SFRP4

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human SFRP4

WB description: Western blot analysis of SFRP4 expression in HepG2 (A), Hela (B) whole cell lysates.

IHC description: Immunohistochemical analysis of SFRP4 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of SFRP4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 6424

Gene ID (Mouse): 20379

Gene ID(Rat): 89803

SwissProt (Human): Q6FHJ7

SwissProt (Mouse): Q9Z1N6

SwissProt (Rat): Q9JLS4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.