Purified Rabbit anti-RPS3A Antibody

SKU: DL97076A

Size 1: 100 ul

Synonyms : FTE1; MFTL; 40S ribosomal protein S3a; v-fos transformation effector protein; Fte-1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RPS3A

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RPS3A

WB description: Western blot analysis of RPS3A expression in MCF7 (A), A549 (B), mouse liver (C), mouse lung (D), rat ovary (E) whole cell lysates.

IHC description: Immunohistochemical analysis of RPS3A staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RPS3A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 6189

Gene ID (Mouse): 20091

Gene ID(Rat): 29288

SwissProt (Human): P61247

SwissProt (Mouse): P97351

SwissProt (Rat): P49242

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.