Purified Rabbit anti-RPLP1 Antibody

SKU: DL97074A

Size 1: 100 ul

Synonyms : RRP1; 60S acidic ribosomal protein P1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to RPLP1

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human RPLP1

WB description: Western blot analysis of RPLP1 expression in SW480 (A), mouse spleen (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates.

IHC description: Immunohistochemical analysis of RPLP1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RPLP1 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 6176

Gene ID (Mouse):

Gene ID(Rat): 100360522; 140661

SwissProt (Human): P05386

SwissProt (Mouse):

SwissProt (Rat): P19944

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.