Purified Rabbit anti-RB1 (pT821) Antibody

SKU: DL98599A

Size 1: 100 ul

Synonyms : Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IH, IF/IC

Background: Rabbit polyclonal antibody to RB1 (pT821)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RB1 (pT821). The exact sequence is proprietary.

WB description: Western blot analysis of RB1 (pT821) expression in HuT78 (A), Myla2059 (B), MCF7 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of RB1 (pT821) staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of RB1 (pT821) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 5925

Gene ID (Mouse): 19645

Gene ID(Rat): 24708

SwissProt (Human): P06400

SwissProt (Mouse): P13405

SwissProt (Rat): P33568

Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.