Purified Rabbit anti-PYGB Antibody

SKU: DL96229A

Size 1: 100 ul

Synonyms : Glycogen phosphorylase, brain form

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to PYGB

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human PYGB

WB description: Western blot analysis of PYGB expression in SW620 (A), HT29 (B), mouse heart (C), mouse lung (D), rat brain (E) whole cell lysates.

IHC description: Immunohistochemical analysis of PYGB staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of PYGB staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Gene ID(Human): 5834

Gene ID (Mouse): 110078

Gene ID(Rat): 25739

SwissProt (Human): P11216

SwissProt (Mouse): Q8CI94

SwissProt (Rat): P53534

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.