Purified Rabbit anti-PRAS40 Antibody

SKU: DL93333A

Size 1: 100 ul

Synonyms : PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PRAS40

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRAS40. The exact sequence is proprietary.

WB description: Western blot analysis of PRAS40 expression in MCF7 (A), human Testis (B) whole cell lysates.

IHC description: Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 84335

Gene ID (Mouse): 67605

Gene ID(Rat):

SwissProt (Human): Q96B36

SwissProt (Mouse): Q9D1F4

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.