Purified Rabbit anti-POLR2E Antibody

SKU: DL93162A

Size 1: 100 ul

Synonyms : DNA-directed RNA polymerases I, II, and III subunit RPABC1; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; RPB5 homolog; XAP4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk

Applications: WB, IHC

Background: Rabbit polyclonal antibody to POLR2E

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR2E. The exact sequence is proprietary.

WB description: Western blot analysis of POLR2E expression in A549 (A), Jurkat (B), HeLa (C), PC12 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of POLR2E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5434

Gene ID (Mouse): 66420

Gene ID(Rat): 690966

SwissProt (Human): P19388

SwissProt (Mouse): Q80UW8

SwissProt (Rat): B0BNE2

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.