Purified Rabbit anti-PKR (pT451) Antibody

SKU: DL92303A

Size 1: 100 ul

Synonyms : PKR; PRKR; Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2; p68 kinase

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC

Background: Rabbit polyclonal antibody to PKR (pT451)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKR (pT451). The exact sequence is proprietary.

WB description: Western blot analysis of PKR (pT451) expression in HeLa PMA-treated (A), Raw264.7 H2O2-treated (B), NIH3T3 LPS-treated (C) whole cell lysates.

IHC description: Immunohistochemical analysis of PKR (pT451) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 5610

Gene ID (Mouse): 19106

Gene ID(Rat):

SwissProt (Human): P19525

SwissProt (Mouse): Q03963

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.