Purified Rabbit anti-OGFR Antibody

SKU: DL98131A

Size 1: 100 ul

Synonyms : Opioid growth factor receptor; OGFr; Protein 7-60; Zeta-type opioid receptor

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to OGFR

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human OGFR

WB description: Western blot analysis of OGFR expression in A431 (A), mouse kidney (B) whole cell lysates.

IHC description: Immunohistochemical analysis of OGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of OGFR staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 11054

Gene ID (Mouse): 72075

Gene ID(Rat):

SwissProt (Human): Q9NZT2

SwissProt (Mouse): Q99PG2

SwissProt (Rat): Q9QXY4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.