Purified Rabbit anti-MK2 (pT222) Antibody

SKU: DL94744A

Size 1: 100 ul

Synonyms : MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B

Applications: WB, IHC

Background: Rabbit polyclonal antibody to MK2 (pT222)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MK2. The exact sequence is proprietary.

WB description: Western blot analysis of MK2 (pT222) expression in U87MG (A), K562 (B), mouse liver (C), rat liver (D) whole cell lysates.

IHC description: Immunohistochemical analysis of MK2 (pT222) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)

Gene ID(Human): 9261

Gene ID (Mouse): 17164

Gene ID(Rat):

SwissProt (Human): P49137

SwissProt (Mouse): P49138

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.