Purified Rabbit anti-MDH2 Antibody

SKU: DL96186A

Size 1: 100 ul

Synonyms : Malate dehydrogenase, mitochondrial

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to MDH2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human MDH2

WB description: Western blot analysis of MDH2 expression in Jurkat (A), A549 (B), mouse liver (C), mouse heart (D) whole cell lysates.

IHC description: Immunohistochemical analysis of MDH2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of MDH2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 4191

Gene ID (Mouse): 17448

Gene ID(Rat): 81829

SwissProt (Human): P40926

SwissProt (Mouse): P08249

SwissProt (Rat): P04636

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.