Purified Rabbit anti-MARK Antibody

SKU: DL93925A

Size 1: 100 ul

Synonyms : MARK1; KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c; MARK2; EMK1; Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b; Par-1b; Par1b; MARK3; CTAK1; EMK2; MAP/microtubule affinity-regulating kinase 3; C-TAK1; cTAK1; Cdc25C-associated protein kinase 1; ELKL motif kinase 2; EMK-2; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Par-1a; Serine/threonine-protein kinase p78; MARK4; KIAA1860; MARKL1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, Z

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to MARK

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARK. The exact sequence is proprietary.

WB description: Western blot analysis of MARK expression in A431 (A), Jurkat (B), mouse heart (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of MARK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of MARK staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 4139; 2011; 4140; 57787

Gene ID (Mouse): 226778; 13728; 17169; 232944

Gene ID(Rat): 117016; 60328; 170577

SwissProt (Human): Q9P0L2; Q7KZI7; P27448; Q96L34

SwissProt (Mouse): Q8VHJ5; Q05512; Q03141; Q8CIP4

SwissProt (Rat): O08678; O08679; Q8VHF0

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.