Purified Rabbit anti-KSR2 Antibody

SKU: DL94487A

Size 1: 100 ul

Synonyms : Kinase suppressor of Ras 2; hKSR2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M

Applications: WB, IHC, IF/IC, IP

Background: Rabbit polyclonal antibody to KSR2

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human KSR2. The exact sequence is proprietary.

WB description: Western blot analysis of KSR2 expression in Hela (A), HEK293T (B), A549 (C), mouse brain (D) whole cell lysates.

IHC description: Immunohistochemical analysis of KSR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of KSR2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 283455

Gene ID (Mouse):

Gene ID(Rat):

SwissProt (Human): Q6VAB6

SwissProt (Mouse): Q3UVC0

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.