Purified Rabbit anti-INTS6 Antibody

SKU: DL96902A

Size 1: 100 ul

Synonyms : DBI1; DDX26; DDX26A; Integrator complex subunit 6; Int6; DBI-1; Protein DDX26; Protein deleted in cancer 1; DICE1

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to INTS6

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human INTS6

WB description: Western blot analysis of INTS6 expression in HL60 (A), SW620 (B), Hela (C) whole cell lysates.

IHC description: Immunohistochemical analysis of INTS6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of INTS6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Gene ID(Human): 26512

Gene ID (Mouse): 18130

Gene ID(Rat):

SwissProt (Human): Q9UL03

SwissProt (Mouse): Q6PCM2

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.