Purified Rabbit anti-IKB alpha (pS32/S36) Antibody

SKU: DL90803A

Size 1: 100 ul

Synonyms : IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P, S

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to IKB alpha (pS32/S36)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKB alpha. The exact sequence is proprietary.

WB description: Western blot analysis of IKB alpha (pS32/S36) expression in HepG2 TNFa-treated (A), Raw264.7 TNFa-treated (B), PC12 TNFa-treated (C) whole cell lysates.

IHC description: Immunohistochemical analysis of IKB alpha (pS32/S36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of IKB alpha (pS32/S36) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 4792

Gene ID (Mouse): 18035

Gene ID(Rat): 25493

SwissProt (Human): P25963

SwissProt (Mouse): Q9Z1E3

SwissProt (Rat): Q63746

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.