Purified Rabbit anti-HNF4 alpha Antibody

SKU: DL95206A

Size 1: 100 ul

Synonyms : HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk, P

Applications: WB, IHC

Background: Rabbit polyclonal antibody to HNF4 alpha

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HNF4 alpha. The exact sequence is proprietary.

WB description: Western blot analysis of HNF4 alpha expression in HT29 (A), rat muscle (B) whole cell lysates.

IHC description: Immunohistochemical analysis of HNF4 alpha staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 3172

Gene ID (Mouse): 15378

Gene ID(Rat): 25735

SwissProt (Human): P41235

SwissProt (Mouse): P49698

SwissProt (Rat): P22449

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.