Purified Rabbit anti-Glycerate Kinase Antibody

SKU: DL94141A

Size 1: 100 ul

Synonyms : HBEBP4; Glycerate kinase; HBeAg-binding protein 4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, Mk

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Glycerate Kinase

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glycerate Kinase. The exact sequence is proprietary.

WB description: Western blot analysis of Glycerate Kinase expression in HepG2 (A), NIH3T3 (B) whole cell lysates.

IHC description: Immunohistochemical analysis of Glycerate Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Glycerate Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 132158

Gene ID (Mouse): 235582

Gene ID(Rat): 684314

SwissProt (Human): Q8IVS8

SwissProt (Mouse): Q8QZY2

SwissProt (Rat): Q0VGK3

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.